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beta-Endorphin - Cat #:MV1003
Product Description polyclonal antibody to human beta-Endorphin conjugated to BSA
beta-EndorphinImmunohistochemical detection of beta-Endorphin in rat ventral lateral periaqueductal grey matter (PAG). Brain was fixed with 4% formaldehyde and cut into 10 μm thick cryostat sections. Tissue was incubated with rabbit polyclonal antibody to beta-Endorphin at 10 μg/mL overnight at 4°C followed by incubation with Donkey anti-rabbit Rhodamine Red conjugated secondary antibodies at 1:200. Tissue was counterstained with DAPI (blue color) to visualize cell nuclei.
Formulation Lyophilized from 10 mM PBS.
Purification Whole IgG
Host Species Rabbit
Unit Size: 50 µg
Immunogen Human beta endorphin conjugated to BSA.
Antigen Location (aa): 1-31
Alternative Names N/A
Accession Number: P01189
Accession Name: COLI_HUMAN
Accession URL:
Function: beta Endorphin is an endogenous opioid peptide that interacts with mu-opioid receptors a, like morphine, produces analgesic effects.
Applications: Recommended for Immunohistochemistry (IHC), Cytochemistry (ICC) and RIA.
Working Dilution for Immunofluorescence (ICC): 5 – 15 µg/mL
Working Dilution for Immunohistochemistry (IHC): 5 – 10 µg/mL
IHC Positive control: Brain (PAG)
Specificity: Confirmed by RIA against the synthetic peptide.
Cross-reactivity: Human; mouse; rat. Beta-endorphin is a highly conserved molecule, so cross-reactivity with other species is expected. Cross-reactivity with other opioid peptides is as follows: with Met-enkephalin 0.03%; with Leu-enkephalin 0.02%; with beta-lipotropin 0.34%.
Reconstitution: Reconstitute in 0.05 mL of PBS (pH 7.4) to achieve an antibody concentration of 1000 µg/mL. Centrifuge to remove any insoluble material.
Storage / Stability: At least 12 months after purchase at 2 - 4°C. After reconstitution, aliquot and store at -20°C for a higher stability and at 4°C with an appropriate antibacterial agent. Avoid freeze-thaw cycles.
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