Somatostatin
(Cat #: MV1012)
| Rabbit polyclonal antibody to the human Somatostatin conjugated to BSA. |
 Immunohistochemical staining of Somatostatin in rat brainstem medulla. 4% paraformaldehyde fixed rat brain 10 μm thick cryostat sections were incubated with rabbit polyclonal antibody to Somatostatin at 10 μg/mL overnight at 4°C followed by incubation with Donkey anti-rabbit Rhodamine Red (pseudo-coloured yellow) conjugated secondary antibodies at 1:200. Cell nuclei were counterstained with DAPI. |
| Formulation |
Lyophilized from 10 mM PBS. |
| Purification |
Whole IgG |
| Host Species |
Rabbit |
| Unit Size: |
50 µg |
| Immunogen |
Somatostatin conjugated to BSA. |
| Sequence: |
AGCKNFFWKTFTSC |
| Antigen Location (aa): |
103-116 |
| Alternative Names |
Growth hormone release-inhibiting factor (SRIF), growth hormone-inhibiting hormone (GHIH). |
| Accession Number: |
P61278 |
| Accession Name: |
(SMS_HUMAN) |
| Accession URL: |
http://www.uniprot.org/uniprot/P61278 |
| Function: |
Somatostatin is secreted by neuroendocrine neurons in hypothalamus and interacts with somatostatin receptors inhibiting secretion of growth hormone. |
| Applications: |
Recommended for Immunohistochemistry (IHC) and Cytochemistry (ICC). |
| Working Dilution for Immunofluorescence (ICC): |
5 – 15 µg/mL |
| Working Dilution for Immunohistochemistry (IHC): |
5 – 10 µg/mL |
| IHC Positive control: |
Brainstem medulla |
| Specificity: |
Confirmed by RIA against the synthetic peptide. |
| Cross-reactivity: |
Human, mouse, rat. |
| Reconstitution: |
Reconstitute in 0.05 mL of PBS (pH 7.4) to achieve an antibody concentration of 1000 µg/mL. Centrifuge to remove any insoluble material |
| Storage / Stability: |
At least 12 months after purchase at 2 - 4°C. After reconstitution, aliquot and store at -20°C for a higher stability and at 4°C with an appropriate antibacterial agent. Avoid freeze-thaw cycles. |
References:
- Hokfelt T, Efendic S, Johansson O, Luft R, Arimura A. Immunohistochemical localization of somatostatin (growth hormone release-inhibiting factor) in the guinea pig brain. Brain Res 1974 Nov 8;80(1):165-9. PMID: 4608681.
- Elde R, Hokfelt T, Johansson O, Schultzberg M, Efendic S, Luft R. Cellular localization of somatostatin Metabolism 1978 Sep;27(9 Suppl 1):1151-9. PMID: 355778.
- Bowker RM. Evidence for the co-localization of somatostatin- and methionine-enkephalin-like immunoreactivities in raphe and gigantocellularis nuclei. Neurosci Lett 1987 Oct 16;81(1-2):75-81. PMID: 2892158.
- Maley BE. Immunohistochemical localization of neuropeptides and neurotransmitters in the nucleus solitarius. Chem Senses 1996 Jun;21(3):367-76. PMID: 8670716.
- Lepousez G, Csaba Z, Bernard V, Loudes C, Videau C, Lacombe J, Epelbaum J, Viollet C. J Comp Neurol. 2010 Jun 1;518(11):1976-94.PMID: 20394054.
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