Neurotensin
- Cat #: MV1011
| Product Description |
Rabbit polyclonal antibody to the human Neurotensin conjugated to BSA. |
 Immunohistochemical staining of Neurotensin in rat brainstem medulla. 4% paraformaldehyde fixed rat brain 10 μm thick cryostat sections were incubated with rabbit polyclonal antibody to Neurotensin at 10 μg/mL overnight at 4°C followed by incubation with Donkey anti-rabbit Rhodamine Red conjugated secondary antibodies at 1:200. Cell nuclei were counterstained with DAPI. |
| Formulation |
Lyophilized from 10 mM PBS. |
| Purification |
Whole IgG |
| Host Species |
Rabbit |
| Unit Size: |
50 µg |
| Immunogen |
Neurotensin conjugated to BSA. |
| Sequence: |
QLYENKPRRPYIL |
| Antigen Location (aa): |
151-163 |
| Alternative Names |
Neuromedin N |
| Accession Number: |
P30990 |
| Accession Name: |
(NEUT_HUMAN) |
| Accession URL: |
http://www.uniprot.org/uniprot/P30990 |
| Function: |
Neurotensin is a neuropeptide which regulates secretion of luteinizing hormone and prolactin. Involved in dopamine neurotransmission. |
| Applications: |
Recommended for Immunohistochemistry (IHC) and Cytochemistry (ICC). |
| Working Dilution for Immunofluorescence (ICC): |
5 – 15 µg/mL |
| Working Dilution for Immunohistochemistry (IHC): |
5 – 10 µg/mL |
| IHC Positive control: |
Brainstem medulla. |
| Specificity: |
Confirmed by RIA against the synthetic peptide. |
| Cross-reactivity: |
Human, mouse, rat. Highly conservative and expected to interact with bovine, feline and monkey. |
| Reconstitution: |
Reconstitute in 0.05 mL of PBS (pH 7.4) to achieve an antibody concentration of 1000 µg/mL. Centrifuge to remove any insoluble material. |
| Storage / Stability: |
At least 12 months after purchase at 2 - 4°C. After reconstitution, aliquot and store at -20°C for a higher stability and at 4°C with an appropriate antibacterial agent. Avoid freeze-thaw cycles. |
References:
- Uhl GR, Goodman RR, Snyder SH. Neurotensin-containing cell bodies, fibers and nerve terminals in the brain stem of the rat: immunohistochemical mapping. Brain Res 1979 May 5;167(1):77-91. PMID: 378326.
- Woodhams PL, Roberts GW, Polak JM, Crow TJ. Distribution of neuropeptides in the limbic system of the rat: the bed nucleus of the stria terminalis, septum and preoptic area. Neuroscience 1983 Apr;8(4):677-703. PMID: 6346134.
- Milner TA, Pickel VM. Neurotensin in the rat parabrachial region: ultrastructural localization and extrinsic sources of immunoreactivity. J Comp Neurol 1986 May 15;247(3):326-43. PMID: 3522659.
- Mai JK, Triepel J, Metz J. Neurotensin in the human brain. Neuroscience 1987 Aug;22(2):499-524. PMID: 3670596.
- Dufourny L, Warembourg M, Jolivet A. Quantitative studies of progesterone receptor and nitric oxide synthase colocalization with somatostatin, or neurotensin, or substance P in neurons of the guinea pig ventrolateral hypothalamic nucleus: an immunocytochemical triple-label analysis.J Chem Neuroanat 1999 Sep;17(1):33-43 PMID: 10569238.
- Reyes R, Valladares F, Gutiérrez R, González M, Bello AR. J Anat. 2008 Jun;212(6):817-26.PMID: 18510508.
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