MVS Pacific logo



Leu-Enkephalin (Cat #: MV1009)
Product Description Rabbit polyclonal antibody to human Met-Enkephalin
Leu-EnkephalinImmunohistochemical detection of Leu-Enkephalin in rat ventral periaqueductal grey matter (PAG). Brain was fixed with 4% formaldehyde and cut into 10 μm thick cryostat sections. Tissue was incubated with rabbit polyclonal antibody to Leu-Enkephalin at 10 μg/mL overnight at 4°C followed by incubation with Donkey anti-rabbit Rhodamine Red (pseudo-coloured yellow) conjugated secondary antibodies at 1:200. Tissue was counterstained with DAPI (blue color) to visualize cell nuclei.
Formulation Lyophilized from 10 mM PBS.
Purification Whole IgG
Host Species Rabbit
Unit Size: 50 µg
Immunogen Leu-Enkephalin conjugated to BSA .
Sequence: YGGFL
Antigen Location (aa): 230 - 234
Alternative Names N/A
Accession Number: P01210
Accession Name: PENK_HUMAN
Accession URL:
Function: Leu-Enkephalin is an endogenous opioid peptide that interacts with opioid receptors and produces analgesic effects.
Applications: Recommended for Immunohistochemistry (IHC), Cytochemistry (ICC) and RIA.
Working Dilution for Immunofluorescence (ICC): 5 – 15 µg/mL
Working Dilution for Immunohistochemistry (IHC): 5 – 10 µg/mL
IHC Positive control: Brain (spinal cord, PAG).
Specificity: Confirmed by RIA against the synthetic peptide.
Cross-reactivity: Human; mouse; rat. Leu-Enkephalin is a highly conserved molecule, so cross-reactivity with other species is expected. Cross-reactivity with other opioid peptides is as follows: with Met-enkephalin 0.93%; with beta-lipotropin 0.01%; with beta-endorphin 0.01%.
Reconstitution: Reconstitute in 0.05 mL of PBS (pH 7.4) to achieve an antibody concentration of 1000 µg/mL. Centrifuge to remove any insoluble material.
Storage / Stability: At least 12 months after purchase at 2 - 4°C. After reconstitution, aliquot and store at -20°C for a higher stability and at 4°C with an appropriate antibacterial agent. Avoid freeze-thaw cycles.
  1. Elde R, Hokfelt T, Johansson O, Terenius L. (1976) Neuroscience, 1:349-351. PMID: 11370520.
  2. Stengaard-Pedersen K, Larsson LI. (1981) Histochemistry, 73(1):89-114. PMID: 6274830.
  3. Wang BL, Larsson LI. (1985) Histochemistry, 83(1):47-56. PMID: 2412988.
  4. Marvizón JC, Chen W, Murphy N. J Comp Neurol. 2009 Nov 1;517(1):51-68.PMID: 19711397.

©2011 MVS Pacific, LLC All rights reserved