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Antibodies


 

Endomorphin-2 (Cat #:MV1007)
Product Description Rabbit polyclonal antibody to synthetic Endomorphin-2
Endo-2 Immunohistochemical staining of Endomorphin 2 in rat ventral lateral periaqueductal grey matter (PAG). 4% paraformaldehyde fixed rat brain 10 μm thick cryostat sections were incubated with rabbit polyclonal antibody to Endomorphin 2 at 10 μg/mL overnight at 4°C followed by incubation with Donkey anti-rabbit Rhodamine Red conjugated secondary antibodies at 1:200. Tissue was counterstained with DAPI (blue color) to visualize cell nuclei.
Formulation Lyophilized from 10 mM PBS.
Purification Whole IgG
Host Species Rabbit
Unit Size: 50 µg
Immunogen Endomorphin-1 conjugated to BSA.
Sequence: YPFF
Antigen Location (aa): 1-4
Alternative Names N/A
Accession Number: Precursor protein is not identified.
Accession Name: Precursor protein is not identified.
Accession URL: Precursor protein is not identified.
Function: Human; mouse; rat. Endomorphin-1 a highly conserved molecule, so cross-reactivity with other species is expected. Cross-reactivity with other opioid peptides is as follows: with Endomorphin-1 < 3%; Met-enkephalin <0.03%; with Leu-enkephalin 0.01%; with beta-endorphin<0.1%
Applications: Recommended for Immunohistochemistry (IHC) and Cytochemistry (ICC).
Working Dilution for Immunofluorescence (ICC): 5 – 15 µg/mL
Working Dilution for Immunohistochemistry (IHC): 5 – 10 µg/mL
IHC Positive control: Brain (spinal cord, PAG, DRG).
Specificity: Confirmed by RIA against the synthetic peptide.
Cross-reactivity: Human; mouse; rat. Endomorphin-1 a highly conserved molecule, so cross-reactivity with other species is expected. Cross-reactivity with other opioid peptides is as follows: with Endomorphin-2 <5%; Met-enkephalin <0.03%; with Leu-enkephalin 0.01%; with beta-endorphin<0.1%
Reconstitution: Reconstitute in 0.05 mL of PBS (pH 7.4) to achieve an antibody concentration of 1000 µg/mL. Centrifuge to remove any insoluble material.
Storage / Stability: At least 12 months after purchase at 2 - 4°C. After reconstitution, aliquot and store at -20°C for a higher stability and at 4°C with an appropriate antibacterial agent. Avoid freeze-thaw cycles.
References:
  1. Zadina JE, et al. A potent and selective endogenous agonist for the mu-opiate receptor. Nature. 1997; 386(6624):499-502. PMID: Zadina JE, Hackler L, Ge LJ, Kastin AJ. Nature. 1997 Apr 3;386(6624):499-502. PMID: 9087409.
  2. Hackler L, et al. Isolation of relatively large amounts of endomorphin-1 and endomorphin-2 from human brain cortex. Peptides. 1997; 18(10):1635-9. PMID: 9437727.
  3. Schreff M, et al. Immunofluorescent identification of endomorphin-2-containing nerve fibers and terminals in the rat brain and spinal cord. Neuroreport. 1998; 20;9(6):1031-4. PMID: 9601662.
  4. Zadina JE.Isolation and distribution of endomorphins in the central nervous system. Jpn J Pharmacol. 2002 Jul;89(3):203-8. Review. PMID: 12184722.
  5. Hui R, Chen T, Li YQ. Neuroscience. 2006;138(1):171-81. PMID: 16360287.
  6. Greenwell TN, Martin-Schild S, Inglis FM, Zadina JE. J Comp Neurol. 2007 Jul 10;503(2):319-33. PMID: 17492626.
  7. Lv BC, Ji GL, Huo FQ, Chen T, Li H, Li YQ. J Chem Neuroanat. 2010 May;39(3):166-74. PMID: 19931378.

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