Atrial Natriuretic Peptide (ANP) - Cat #:MV1002
| Rabbit polyclonal antibody to the rat Atrial Natriuretic Peptide (ANP) conjugated to BSA. |
 Immunohistochemical staining of Atrial Natriuretic Peptide (ANP) in rat convoluted tubules in kidney. 4% paraformaldehyde fixed rat kidney 10 μm thick cryostat sections were incubated with rabbit polyclonal antibody to of Atrial Natriuretic Peptide (ANP) at 10 μg/mL overnight at 4°C followed by incubation with Donkey anti-rabbit Rhodamine Red (pseudo-coloured yellow) conjugated secondary antibodies at 1:200. |
| Formulation |
Lyophilized from 10 mM PBS. |
| Purification |
Whole IgG |
| Host Species |
Rabbit |
| Unit Size: |
50 µg |
| Immunogen |
Atrial natriuretic peptide (ANP) conjugated to BSA. |
| Sequence: |
SLRRSSCFGGRIDRIGAQSGLGCNSFRY |
| Antigen Location (aa): |
123-150 |
| Alternative Names |
Atrial natriuretic factor (ANF). |
| Accession Number: |
P01161 |
| Accession Name: |
(ANF_RAT) |
| Accession URL: |
http://www.uniprot.org/uniprot/P01161 |
| Function: |
Atrial Natriuretic Peptide (ANP) is produced by cardiomyocites and is secreted into the bloodstream. ANP acts on convoluted tubules in kidney reducing sodium absorption which results in reducing the blood pressure. |
| Applications: |
Recommended for Immunohistochemistry (IHC) and Cytochemistry (ICC). |
| Working Dilution for Immunofluorescence (ICC): |
5 – 15 µg/mL |
| Working Dilution for Immunohistochemistry (IHC): |
5 – 10 µg/mL |
| IHC Positive control: |
Heart, kidney. |
| Specificity: |
Confirmed by RIA against the synthetic peptide. |
| Cross-reactivity: |
Human; mouse; rat. Highly conservative and expected to interact with feline, canine, porcine, bovine, and equine ANP/ANF. |
| Reconstitution: |
Reconstitute in 0.05 mL of PBS (pH 7.4) to achieve an antibody concentration of 1000 µg/mL. Centrifuge to remove any insoluble material. |
| Storage / Stability: |
At least 12 months after purchase at 2 - 4°C. After reconstitution, aliquot and store at -20°C for a higher stability and at 4°C with an appropriate antibacterial agent. Avoid freeze-thaw cycles. |
References:
- Maley BE. Immunohistochemical localization of neuropeptides and neurotransmitters in the nucleus solitarius. Chem Senses 1996 Jun;21(3):367-76. PMID: 8670716.
- Jougasaki M, Wei CM, Aarhus LL, Heublein DM, Sandberg SM, Burnett JC Jr. Renal localization and actions of adrenomedullin: a natriuretic peptide. Am J Physiol 1995 Apr;268(4 Pt 2):F657-63. PMID: 7733322.
- Kawaguchi S, Uchida K, Ito T, Kozuka M, Shimonaka M, Mizuno T, Hirose S. Immunohistochemical localization of atrial natriuretic peptide receptor in bovine kidney and lung. J Histochem Cytochem 1989 Nov;37(11):1739-42. PMID: 2553804.
- Chapeau C, Gutkowska J, Schiller PW, Milne RW, Thibault G, Garcia R, Genest J, Cantin M. Localization of immunoreactive synthetic atrial natriuretic factor (ANF) in the heart of various animal species. J Histochem Cytochem 1985 Jun;33(6):541-50 PMID: 3158698.
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