Adrenocorticotropic Hormone (ACTH)
- Cat #:MV1001
| Product Description |
Rabbit polyclonal antibody to Adrenocorticotropic hormone (ACTH) |
 Immunohistochemical detection of ACTH in rat hypothalamus. Rat brain was fixed with 4% formaldehyde and cut into 10 μm thick cryostat sections. Tissue was incubated with rabbit polyclonal antibody to ACTH at 5 μg/mL overnight at 4°C followed by incubation with Donkey anti-rabbit Rhodamine Red conjugated secondary antibodies at 1:200. Tissue was counterstained with DAPI (blue color) to visualize cell nuclei. |
| Formulation |
Lyophilized from 10 mM PBS. |
| Purification |
Whole IgG |
| Host Species |
Rabbit |
| Unit Size: |
50 µg |
| Immunogen |
Porcine ACTH (Adrenocorticotropic hormone) conjugated to BSA. |
| Sequence: |
SYSMEHFRWGKPVGRKRRPVKVYP |
| Antigen Location (aa): |
1-24 |
| Alternative Names |
Corticotropin |
| Accession Number: |
P01192 |
| Accession Name: |
COLI_PIG |
| Accession URL: |
http://www.uniprot.org/uniprot/P01192 |
| Function: |
ACTH is produced by the pituitary gland from which it is secreted and stimulates release of cortisol by adrenal glands. |
| Applications: |
Recommended for Immunohistochemistry (IHC), Cytochemistry (ICC) and RIA. |
| Working Dilution for Immunofluorescence (ICC): |
5 – 15 µg/mL |
| Working Dilution for Immunohistochemistry (IHC): |
5 – 10 µg/mL |
| IHC Positive control: |
Brain (pituitary, hypothalamus) |
| Specificity: |
Confirmed by RIA against the synthetic peptide. |
| Cross-reactivity: |
Human; mouse; rat ACTH. ACTH is a highly conserved molecule, so cross-reactivity with other species is expected. |
| Reconstitution: |
Reconstitute in 0.05 mL of PBS (pH 7.4) to achieve an antibody concentration of 1000 µg/mL. Centrifuge to remove any insoluble material. |
| Storage / Stability: |
At least 12 months after purchase at 2 - 4°C. After reconstitution, aliquot and store at -20°C for a higher stability and at 4°C with an appropriate antibacterial agent. Avoid freeze-thaw cycles. |
References
- Brozman M. Histochemical localization of ACTH and TSH in the human hypophysis. Acta Histochem 1967;26(2):261-70. PMID: 4298472.
- Watson SJ, Richard CW 3d, Barchas JD. Adrenocorticotropin in rat brain: immunocytochemical localization in cells and axons. Science 1978 Jun 9;200(4346):1180-2. PMID: 206967.
- Pelletier G, Desy L. Localization of ACTH in the human hypothalamus. Cell Tissue Res 1979 Feb 28;196(3):525-30. PMID: 222452.
- Childs G, Unabia G. Application of the avidin-biotin-peroxidase complex (ABC) method to the light microscopic localization of pituitary hormones. J Histochem Cytochem 1982 Jul;30(7):713-6. PMID: 6286753.
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