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Antibodies


 

Adrenocorticotropic Hormone (ACTH) - Cat #:MV1001
Rabbit polyclonal antibody to Adrenocorticotropic hormone (ACTH)
ACTHImmunohistochemical detection of ACTH in rat hypothalamus. Rat brain was fixed with 4% formaldehyde and cut into 10 μm thick cryostat sections. Tissue was incubated with rabbit polyclonal antibody to ACTH at 5 μg/mL overnight at 4°C followed by incubation with Donkey anti-rabbit Rhodamine Red conjugated secondary antibodies at 1:200. Tissue was counterstained with DAPI (blue color) to visualize cell nuclei.
Formulation Lyophilized from 10 mM PBS.
Purification Whole IgG
Host Species Rabbit
Unit Size: 50 µg
Immunogen Porcine ACTH (Adrenocorticotropic hormone) conjugated to BSA.
Sequence: SYSMEHFRWGKPVGRKRRPVKVYP
Antigen Location (aa): 1-24
Alternative Names Corticotropin
Accession Number: P01192
Accession Name: COLI_PIG
Accession URL: http://www.uniprot.org/uniprot/P01192
Function: ACTH is produced by the pituitary gland from which it is secreted and stimulates release of cortisol by adrenal glands.
Applications: Recommended for Immunohistochemistry (IHC), Cytochemistry (ICC) and RIA.
Working Dilution for Immunofluorescence (ICC): 5 – 15 µg/mL
Working Dilution for Immunohistochemistry (IHC): 5 – 10 µg/mL
IHC Positive control: Brain (pituitary, hypothalamus)
Specificity: Confirmed by RIA against the synthetic peptide.
Cross-reactivity: Human; mouse; rat ACTH. ACTH is a highly conserved molecule, so cross-reactivity with other species is expected.
Reconstitution: Reconstitute in 0.05 mL of PBS (pH 7.4) to achieve an antibody concentration of 1000 µg/mL. Centrifuge to remove any insoluble material.
Storage / Stability: At least 12 months after purchase at 2 - 4°C. After reconstitution, aliquot and store at -20°C for a higher stability and at 4°C with an appropriate antibacterial agent. Avoid freeze-thaw cycles.
References
  1. Brozman M. Histochemical localization of ACTH and TSH in the human hypophysis. Acta Histochem 1967;26(2):261-70. PMID: 4298472.
  2. Watson SJ, Richard CW 3d, Barchas JD. Adrenocorticotropin in rat brain: immunocytochemical localization in cells and axons. Science 1978 Jun 9;200(4346):1180-2. PMID: 206967.
  3. Pelletier G, Desy L. Localization of ACTH in the human hypothalamus. Cell Tissue Res 1979 Feb 28;196(3):525-30. PMID: 222452.
  4. Childs G, Unabia G. Application of the avidin-biotin-peroxidase complex (ABC) method to the light microscopic localization of pituitary hormones. J Histochem Cytochem 1982 Jul;30(7):713-6. PMID: 6286753.

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