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Caspase 3 (C-terminal; Cat #: AA119)
Rabbit polyclonal antibody to C-terminal region of Caspase 3
Caspase 3 C-termImmunohistochemical detection of Caspase 3 in apoptosis induced Jurkat cells. Cells were fixed with 1% formaldehyde incubated with rabbit polyclonal antibody to C-terminal region of Caspase 3 at 8 μg/mL overnight at 4°C followed by incubation with Donkey anti-rabbit Rhodamine Red conjugated secondary antibodies at 1:200 dilution. Nuclei were counterstained with DAPI (blue). Caspase 3 C-term WB
Formulation Lyophilized powder
Purification Affinity purified
Host Species Rabbit
Unit Size: 50 µg
Immunogen Synthetic peptide
Alternative Names Apopain, Cysteine protease CPP32 (CPP-32), protein Yama, SREBP cleavage activity 1 (SCA-1)
Accession Number: P42574
Gene Symbol CASP3
Accession URL:
Caspase 3 is a heterotetramer of two heterodimers arranged in anti-parallel orientation. Casapse 3 activates caspases 6 and 7 and this sequential activation leads to apoptosis. CASP3 is a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Caspase 3 participates in cleavage of Amyloid beta 4A precursor protein which is implicated with neuronal death Alzheimer's disease. Caspase 3 in turn is processed by caspases 8, 9 and 10.
Applications: Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blotting (WB).
Working Dilution for Immunofluorescence (ICC): 2– 15 µg/mL
Working Dilution for Immunohistochemistry (IHC): 5 – 10 µg/mL
Working Dilution for Western Blottin (WB): 1 µg/mL
IHC Positive control: Cells (HeLA, Jurkat) treated with agents that induce apoptosis such as antibiotic AM-2282 or staurosporin (STS). Spleen, kidney and heart.
Specificity: Confirmed by WB.
Reactivity: Human
Reconstitution: Reconstitute in 0.05 mL of PBS (pH 7.4) to achieve an antibody concentration of 1000 µg/mL. Centrifuge to remove any insoluble material.
Storage / Stability: At least 12 months after purchase at 2 - 4°C. After reconstitution, aliquot and store at -20°C for a higher stability and at 4°C with an appropriate antibacterial agent. Avoid freeze-thaw cycles.
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