| Product Description | Rabbit polyclonal antibody to the human Somatostatin conjugated to BSA. |
|---|---|
 Immunohistochemical staining of Somatostatin in rat brainstem medulla. 4% paraformaldehyde fixed rat brain 10 μm thick cryostat sections were incubated with rabbit polyclonal antibody to Somatostatin at 10 μg/mL overnight at 4°C followed by incubation with Donkey anti-rabbit Rhodamine Red (pseudo-coloured yellow) conjugated secondary antibodies at 1:200. Cell nuclei were counterstained with DAPI. | |
| Formulation | Lyophilized from 10 mM PBS. |
| Purification | Whole IgG |
| Host Species | Rabbit |
| Unit Size: | 50 µg |
| Immunogen | Somatostatin conjugated to BSA. |
| Sequence: | AGCKNFFWKTFTSC |
| Antigen Location (aa): | 103-116 |
| Alternative Names | Growth hormone release-inhibiting factor (SRIF), growth hormone-inhibiting hormone (GHIH). |
| Accession Number: | P61278 |
| Accession Name: | (SMS_HUMAN) |
| Accession URL: | http://www.uniprot.org/uniprot/P61278 |
| Function: | Somatostatin is secreted by neuroendocrine neurons in hypothalamus and interacts with somatostatin receptors inhibiting secretion of growth hormone. |
| Applications: | Recommended for Immunohistochemistry (IHC) and Cytochemistry (ICC). |
| Working Dilution for Immunofluorescence (ICC): | 5 – 15 µg/mL |
| Working Dilution for Immunohistochemistry (IHC): | 5 – 10 µg/mL |
| IHC Positive control: | Brainstem medulla |
| Specificity: | Confirmed by RIA against the synthetic peptide. |
| Cross-reactivity: | Human, mouse, rat. |
| Reconstitution: | Reconstitute in 0.05 mL of PBS (pH 7.4) to achieve an antibody concentration of 1000 µg/mL. Centrifuge to remove any insoluble material |
| Storage / Stability: | At least 12 months after purchase at 2 - 4°C. After reconstitution, aliquot and store at -20°C for a higher stability and at 4°C with an appropriate antibacterial agent. Avoid freeze-thaw cycles. |
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