 Immunohistochemical staining of Gastrin in G cells in rat gastric glands. Tissue was fixed with 4% formaldehyde and cut into 10 μm thick cryostat sections, and incubated with rabbit polyclonal antibody to Gastrin at 15 μg/mL overnight at 4°C followed by incubation with Donkey anti-rabbit Rhodamine Red conjugated secondary antibodies at 1:200. DAPI (blue color) was used to counterstain cell nuclei. |
| Formulation | Lyophilized from 10 mM PBS. |
| Purification | Whole IgG |
| Host Species | Rabbit |
| Unit Size: | 50 µg |
| Immunogen | Gastrin conjugated to BSA. |
| Sequence: | QGPWLEEEEE |
| Antigen Location (aa): | 76-85 |
| Alternative Names | N/A |
| Accession Number: | P01350 |
| Accession Name: | GAST _HUMAN |
| Accession URL: | http://www.uniprot.org/uniprot/P01350 |
| Function: | Gastrin is a 101-amino acid hormone produced by G cells of the duodenum, stomach and pancreas. It stimulates secretion of HCl by parietal cells of the stomach. Gastrin is also secreted into bloodstream. |
| Applications: | Recommended for Immunohistochemistry (IHC), Cytochemistry (ICC) and RIA. |
| Working Dilution for Immunofluorescence (ICC): | 5 – 15 µg/mL |
| Working Dilution for Immunohistochemistry (IHC): | 5 – 10 µg/mL |
| IHC Positive control: | Brain (pituitary, hypothalamus) |
| Specificity: | Confirmed by RIA against the synthetic peptide. |
| Cross-reactivity: | Human, mouse, rat. |
| Reconstitution: | Reconstitute in 0.05 mL of PBS (pH 7.4) to achieve an antibody concentration of 1000 µg/mL. Centrifuge to remove any insoluble materia. |
| Storage / Stability: | At least 12 months after purchase at 2 - 4°C. After reconstitution, aliquot and store at -20°C for a higher stability and at 4°C with an appropriate antibacterial agent. Avoid freeze-thaw cycles. |
References- Vanderhaeghen JJ, Lotstra F, De Mey J, Gilles C Immunohistochemical localization of cholecystokinin- and gastrin-like peptides in the brain and hypophysis of the rat. Proc Natl Acad Sci U S A 1980 Feb;77(2):1190-4. PMID: 6987667.
- Helen P, Panula P, Yang HY, Hervonen A, Rapoport SI. Location of substance P-, bombesin-gastrin-releasing peptide [Met5]enkephalin- and [Met5]enkephalin-Arg6-Phe7-like immunoreactivities in adult human sympathetic ganglia. Neuroscience 1984 Jul;12(3):907-16. PMID: 6206441.
- Rix EW, Feurle GE, Carraway RE. Co-localization of xenopsin and gastrin immunoreactivity in gastric antral G-cells. Histochemistry 1986;85(2):135-8. PMID: 3528076.
- Bolkent S, Yilmazer S. Alterations in gastrin cells induced by short-term omeprazole treatment in the rat antrum: an immunocytochemical and in situ hybridization study. Histochem J 1997 Jun;29(6):479-85. PMID: 9248855.
- Sentani K, Oue N, Noguchi T, et al.,Immunostaining of gastric cancer with neuroendocrine differentiation: Reg IV-positive neuroendocrine cells are associated with gastrin, serotonin, pancreatic polypeptide and somatostatin. Pathol Int. 2010 Apr;60(4):291-7. PMID: 20403031.
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