| Product Description | Rabbit polyclonal antibody to synthetic Endomorphin-2 |
|---|---|
 Immunohistochemical staining of Endomorphin 2 in rat ventral lateral periaqueductal grey matter (PAG). 4% paraformaldehyde fixed rat brain 10 μm thick cryostat sections were incubated with rabbit polyclonal antibody to Endomorphin 2 at 10 μg/mL overnight at 4°C followed by incubation with Donkey anti-rabbit Rhodamine Red conjugated secondary antibodies at 1:200. Tissue was counterstained with DAPI (blue color) to visualize cell nuclei. | |
| Formulation | Lyophilized from 10 mM PBS. |
| Purification | Whole IgG |
| Host Species | Rabbit |
| Unit Size: | 50 µg |
| Immunogen | Endomorphin-1 conjugated to BSA. |
| Sequence: | YPFF |
| Antigen Location (aa): | 1-4 |
| Alternative Names | N/A |
| Accession Number: | Precursor protein is not identified. |
| Accession Name: | Precursor protein is not identified. |
| Accession URL: | Precursor protein is not identified. |
| Function: | Human; mouse; rat. Endomorphin-1 a highly conserved molecule, so cross-reactivity with other species is expected. Cross-reactivity with other opioid peptides is as follows: with Endomorphin-1 < 3%; Met-enkephalin <0.03%; with Leu-enkephalin 0.01%; with beta-endorphin<0.1% |
| Applications: | Recommended for Immunohistochemistry (IHC) and Cytochemistry (ICC). |
| Working Dilution for Immunofluorescence (ICC): | 5 – 15 µg/mL |
| Working Dilution for Immunohistochemistry (IHC): | 5 – 10 µg/mL |
| IHC Positive control: | Brain (spinal cord, PAG, DRG). |
| Specificity: | Confirmed by RIA against the synthetic peptide. |
| Cross-reactivity: | Human; mouse; rat. Endomorphin-1 a highly conserved molecule, so cross-reactivity with other species is expected. Cross-reactivity with other opioid peptides is as follows: with Endomorphin-2 <5%; Met-enkephalin <0.03%; with Leu-enkephalin 0.01%; with beta-endorphin<0.1% |
| Reconstitution: | Reconstitute in 0.05 mL of PBS (pH 7.4) to achieve an antibody concentration of 1000 µg/mL. Centrifuge to remove any insoluble material. |
| Storage / Stability: | At least 12 months after purchase at 2 - 4°C. After reconstitution, aliquot and store at -20°C for a higher stability and at 4°C with an appropriate antibacterial agent. Avoid freeze-thaw cycles. |
References:
| |
