| Product Description | Rabbit polyclonal antibody to N-terminal portion of human beta Lipotropin conjugated to thyroglobulin |
 Immunohistochemical staining of beta-Lipotropin in mouse brain. 4% paraformaldehyde fixed rat brain 10 μm thick cryostat sections were incubated with rabbit polyclonal antibody to beta-Lipotropin at 10 μg/mL overnight at 4°C followed by incubation with Donkey anti-rabbit Rhodamine Red conjugated secondary antibodies at 1:200. Tissue was counterstained with DAPI (blue color) to visualize cell nuclei. | |
| Formulation | Lyophilized from 10 mM PBS. |
| Purification | Whole IgG |
| Host Species | Rabbit |
| Unit Size: | 50 µg |
| Immunogen | human beta Lipotropin conjugated to thyroglobulin. |
| Antigen Location (aa): | 179 - 267 |
| Alternative Names | beta-LPH |
| Accession Number: | P01189 |
| Accession Name: | COLI_HUMAN |
| Accession URL: | http://www.uniprot.org/uniprot/P01189 |
| Function: | beta-Lipotropin is a 90-amino acid polypeptide that is the carboxy-terminal fragment of POMC. It stimulates melanocytes to produce melanin, and can also be cleaved into smaller peptides including opioid peptides: gamma-lipotropin, alpha-MSH, beta-MSH, gamma-MSH, alpha-endorphin, beta-endorphin, gamma-endorphin and met-enkephalin. |
| Applications: | Recommended for Immunohistochemistry (IHC) and Cytochemistry (ICC). |
| Working Dilution for Immunofluorescence (ICC): | 5 – 15 µg/ml |
| Working Dilution for Immunohistochemistry (IHC): | 5 – 10 µg/ml |
| IHC Positive control: | Brain |
| Specificity: | Confirmed by RIA against the synthetic peptide. |
| Cross-reactivity: | Human; mouse; rat. Beta-endorphin is a highly conserved molecule, so cross-reactivity with other species is expected. Cross-reactivity with other opioid peptides is as follows: with Met-enkephalin 0.03%; with Leu-enkephalin 0.02%; with beta-lipotropin 0.34%. |
| Reconstitution: | Reconstitute in 0.05 ml of PBS (pH 7.4) to achieve an antibody concentration of 1000 µg/ml. Centrifuge to remove any insoluble material. |
| Storage / Stability: | At least 12 months after purchase at 2 - 4°C. After reconstitution, aliquot and store at -20°C for a higher stability and at 4°C with an appropriate antibacterial agent. Avoid freeze-thaw cycles. |
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